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CURRICULUM VITAE María Yoanna Eissler Parada, Doctor in Oceanography Working address: Gran Bretaña 1111, Fac Ciencias, Instituto de Química y Bioquímica, Playa Ancha, Valparaíso. Work phone: 56-32-2508063, Cell phone: 09-9977555 E-mail address: yeissler@gmail.com Web pages: www.cigren.cl, www.institutodequimicaybioquimica.cl 1- BRIEF SUMMARY I have experience in the area of Marine Biology and Oceanography, including the specific areas of ecology in marine systems, plankton and microbiology. I have worked either in Chile or abroad, doing research in different projects financed by National and International grants. I carried out a post-doc at the University of Concepción in the Center of Oceanography of the South Eastern Pacific (COPAS) and another post-doc in USA at the Smithsonian Environmental Research Center (SERC). I currently work as an assistant professor at the University of Valparaíso, doing research and teaching. The courses are related to environmental science such as, Marine Chemistry, Applied Biochemistry and Environmental Chemistry among other. Through my Doctorate I carried out a stay of 10 months at the Swedish Meteorological and Hydrological Institute, Gothenburg, Sweden with a scholarship given by the Swedish Institute. I have participated in several specialization courses and have presented my research work in different meetings and workshops either national or international I also have publications related with marine microbiology and ecology of microplankton. Along my carrier I have participated in many national and international oceanographic campaigns, and I have participated in field campaigns to Salar Huasco, 1st region, Chile, having in general a broad experience on field and lab work. 2- VOCATIONAL TRAINING o Currently working as associated professor, Faculta de Ciencias, Instituto de Química y Bioquímica, Universidad de Valparaíso, since May 2009. o Researcher, CIEN Austral - Universidad Austral de Chile, Puerto Montt, Chile, 2008-2009 o Post-doc at Smithsonian Environmental Research Center, Edgewater, Maryland, USA, 2004-2007. o Post-doc at Center Fondap-COPAS, University of Concepción, Concepción, 2002-2004. o Doctor in Oceanography, Department of Oceanography, University of Concepción, Concepción, 2002. o Stay related to Marine Microbiology at Swedish Meteorological and Hydrological Institute, Gothenburg, Sweden, 2000. o Course “Topics on Coastal Geomorphology“, Centro EULA-Chile, University of Concepción, Concepción, 1995. o Course "III International course of Marine Phytoplankton with special stress in Bacillariophytas", P. Universidad Católica de Puerto Rico, Ponce, Puerto Rico. 1995. o Course "Isolation, Identification and Biosynthesis of Marine Biotoxins of Red Tides", University of Concepción, Concepción, Chile, 1994. o Marine Biologist, University of Concepción, Concepción, Chile, 1992. o Graduated in Marine Biology, University of Concepción, Concepción, Chile, 1991. 3- PARTICIPATION INTERNATIONAL RESEARCH PROJECTS Project Cornell University, USA (2013-2014): Building Cornell – Chile collaborations in marine science and metagenomics through exploration of viruses in the Chilean sea urchin Loxechinus alba. Collaborator Researcher. MOVE project, NSF Grand, (2004-2007) “Intra-annual diversity and dynamics of Chesapeake Bay virioplankton” Postdoctoral position, Smithsonian Environmental Research Center, Edgewater, Maryland, USA. Project Wallenberg Foundation (2000), “Host specificity and algal-virus interaction”, 10 months appointment as invited investigator, Swedish Meteorological and Hydrological Institute, Gothenburg, Sweden. 4- PARTICIPATION NATIONAL RESEARCH PROJECTS FIPA Nº 2014-60 (2015– 2017) "Determinación de factores epidemiológicos de riesgo en la presentación clínica de la enfermedad Necrosis Pancreática Infecciosa“. Principal investigador. Proyecto Fondecyt 1140179 (2014-2016) “The Rare Biosphere in High Altitude Wetlands: Funtional Role and The Limits of Life”. Co-PI. Red Nacional de Laboratorios de Referencia para el Diagnóstico de Patógenos Intracelulares en la Industria del Salmón (2013-2014). Subpesca 4728-43-LP13 “Identificación de Cepas y Nuevas Variantes de IPNV y Evaluación del Impacto de éstas en Atención a su Distribución Geográfica y Características de Cuadros Clínicos”, Director alterno, Universidad de Valparaíso. Research project (2011-2013) “Aislamiento y caracterización de virus que infectan a microalgas”, DIPUV-REG. Nº 27/2009, principal investigador, Universidad de Valparaíso. Red Nacional de Laboratorios de Referencia para el Diagnóstico de Patógenos Intracelulares en la Industria del Salmón (2012-2013). Sernapesca, “Evaluación y estandarización de métodos diagnósticos para la determinación del virus de la necrosis pancreática infecciosa (IPNV)”, Co-Investigator, Universidad de Valparaíso. Project Innova Chile/CORFO, 05 CT6IPD-22 (2008-2011), Red Nacional de Laboratorios de Referencia para el Diagnóstico de Patógenos Intracelulares en la Industria del Salmón, Co-Investigator, Universidad de Valparaíso. Proyecto Investigación (2010) “Evaluación Hidrográfica y Epidemiológica del Fiordo Cupquelán, Región Aysén (Primera Etapa)”, Financiamiento Salmones Cupquelán S.A., principal investigador, Universidad de Valparaíso. Project FONDAP-COPAS Center for Oceanography (2002-2004). “Plankton communities: structure, trophic and metabolic processes”, Postdoctoral position, University of Concepción, Marine Biology Station, Dichato. Project FONDAP/CONICYT (1997-1999). “Study of the Humbolt Current System and Benthic-Pelagic coupling” Co-investigator, Department of Oceanography, University of Concepción, Concepción, Chile Project SECTORIAL/FONDECYT (1996-1998). “Primary Production and its destiny through the food web in the pelagic ecosystem off Antofagasta area 23°S”, Co-investigator, Department of Oceanography, University of Concepción, Concepción, Chile. Project SAREC/CONICYT, JGOFS (1991-1997). “JGOFS The Joint Global Ocean Flux Study”, Co-investigator, Department of Oceanography, University of Concepción, Concepción, Chile Project UNDP (1992). "Biological Patterns for Trachurus murphy Capture". Co-investigator, Department of Oceanography, University of Concepción, Concepción, Chile. Project IRDC Canada (1991). "Plankton Ecology". Co-investigator, Department of Oceanography, University of Concepción, Concepción, Chile 5- PUBLICATIONS Tapia, D, Eissler, Y, P. Torres, E. Jorquera, J. C. Espinoza, J. Kuznar (2015) Detection and phylogenetic analysis of infectious pancreatic necrosis virus in Chile. Diseases of aquatic organisms. Vol. 116: 173– 184, 2015 doi: 10.3354/dao02912 Jorquera E; Morales P; Tapia D; Torres P; Eissler Y; Espinoza JC; Conejeros P; Kuznar J. (2016). Chilean IPNV isolates: robustness analysis of PCR detection Journal of Biotechnology. Molina V, CE Morales, L Farías, M Cornejo, M Graco, Y Eissler & LA Cuevas (2012). Potential contribution of planktonic components to ammonium cycling in the coastal area off central-southern Chile during non-upwelling conditions. Progress in Oceanography Volumes 92-95, Special Issue: 4349. DOI: 10.1016/j.pocean.2011.07.006 Eissler Y, MS Pavlov, P Conejeros, JC Espinoza & J Kuznar (2011). Detection and quantification of Chilean strains of infectious pancreatic necrosis virus by real-time RT-PCR assays using segment B as a target. Latin American Journal of Aquatic Research 39(3): 544-552. DOI: 10.3856/vol39-issue3-fulltext-14 Pavlov MS, Y Eissler, JC Espinoza, D Garland & J Kuznar (2011). Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects. Electronic Journal of Biotechnology 14(1): 1-8. DOI: 10.2225/vol14-issue1-fulltext-11 Eissler Y, J Letelier, LA Cuevas, CE Morales & R Escribano (2010). The microbial community in the coastal upwelling system off Concepción, Chile, 36°S, 2002-2003 period. Revista de Biología Marina y Oceanografía 45(1): 1-18. Eissler Y, K Wang, F Chen, KE Wommack & DW Coats (2009). Ultrastructural characterization of the lytic cycle of an intranuclear virus infecting the diatom Chaetoceros cf. wighamii (Bacillariophyceae) from Chesapeake Bay, USA. Journal of Phycology 45(4): 787-797. DOI: 10.1111/j.15298817.2009.00705.x Molina V, L Farías, Y Eissler, LA Cuevas, CE Morales & R Escribano (2005). Ammonium cycling under a strong oxygen gradient associated with the Oxygen Minimum Zone off northern Chile (~23°S). Marine Ecology Progress Series 288: 35-43. DOI: 10.3354/meps288035 Eissler Y, E Sahlsten & RA Quiñones (2003). Effects of virus infection on respiration rates of marine phytoplankton and microplankton communities. Marine Ecology Progress Series 262: 71-80. DOI: 10.3354/meps262071 Eissler Y & RA Quiñones (2003). The effect of viral concentrate addition on the respiration rate of Chaetoceros gracilis cultures and microplankton from a shallow bay (Coliumo, Chile). Journal of Plankton Research 25(8): 927-938. DOI: 10.1093/plankt/25.8.927 Eissler Y & RA Quiñones (1999). Microplanktonic respiration off northern Chile during El Niño 19971998. Journal of Plankton Research 21(12): 2263-2283. DOI: 10.1093/plankt/21.12.2263 6- PUBLISHED ASTRACTS INTERNATIONAL MEETINGS (PRESENTED SINCE 2000 LISTED SINCE 2005) Eissler Y., Wang K. and Coats D. W. (2007). Ultrastructural characterization of the lytic cycle of an intra-nuclear virus of Chaetoceros wighamii from Chesapeake Bay, USA. 2007 PSA-ISPO Meeting, August, Providence, Rhode Island, USA. Eissler, Y., Wang, K., Feng, F., Coats, D. W. (2006) Molecular characterization of Chaetoceros cf gracilis virus isolated from Chesapeake Bay, USA. ASLO summer meeting conference abstracts pag, 35, Victoria, Canada. Salhsten, E., Eissler, Y. (2006) Virus and microalgae in the Baltic Sea. ASLO summer meeting conference abstracts pag, 105, Victoria, Canada. Eissler, Y., Coats, D. W. (2006) Characterization of Chaetoceros cf gracilis virus isolated from Chesapeake Bay, USA. SCOR workshop on methods, University of British Columbia, Vancouver, Canada. Eissler, Y., Coats, D. W. (2005) Characterization of phytoplankton-viral systems from Chesapeake Bay, USA. ASLO summer meeting conference abstracts pag, 46, Santiago de Compostela, Spain. 6- PUBLISHED ABSTRACTS NATIONAL MEETINGS (PRESENTED SINCE 1991, LISTED SINCE 2010) Gálvez, M. J., González, Jose., Dorador, Cristina., Eissler, Yoanna., Fernández, Camila., Hengst, Martha., Hernández, Klaudia., Cornejo, Marcela., Molina, Verónica. Microbioma de ambientes acuaticos característicos de humedales andinos durante un periodo seco y lluvioso. (Microbiome from aquatic environments characteristics from Andean wetlands during a dry and wet period). XXXVII Congreso Sociedad de Microbiología de Chile. La Serena, 1-4 de Diciembre de 2015. Tapia, D., Eissler, Y., Espinoza, J C., Kuznar, J. Ensayo de comparación inter-laboratorios para evaluar las técnicas utilizadas en el diagnóstico del virus IPN. XXXVI Congreso Sociedad de Microbiología de Chile. La Serena, 2-5 de Diciembre de 2014. Y. Eissler, V. Molina, C. Dorador, D. Tapia. Presencia de virus en micro ambientes diversos en el Salar de Huasco y su rol potencial sobre la vida microbiana. Presence of virus in micro diverse environments in Salar de Huasco and its potential role on microbial life. XXXV Congreso Sociedad de Microbiología de Chile. Maitencillo, 26-30 de Noviembre de 2013. Eissler, Y, Tapia D, Torres P, Espinoza JC, Conejeros P, Kuznar J.. Comparación de Métodos de Diagnóstico para la Detección e Identificación del Virus de la Necrosis Pancreática Infecciosa (IPNV) en Salmonidos. Comparison of diagnostic methods to detection and identification of infectious pancreatic necrosis virus (IPNV) in salmonids. XXXV Congreso Sociedad de Microbiología de Chile. Maitencillo, 26-30 de Noviembre de 2013. P. I Torres, Y. Eissler, M.S. Pavlov, P. Conejeros, J.C. Espinoza, D. Tapia & J. Kuznar. Caracterización filogenética de aislados Chilenos del virus IPN en base a una región codificante del segmento VP2. XXXIII Congreso de Ciencias del Mar, Antofagasta, 27-30 de Mayo de 2013, pág. 14. D.Tapia, Y. Eissler, J. C. Espinoza, P. Conejeros, E. Jorquera & J. Kuznar. Comparación de metodologías para la detección del virus de la necrosis pancreática infecciosa (IPNV). XXXIII Congreso de Ciencias del Mar, Antofagasta, 27-30 de Mayo de 2013, pág. 17. Eissler Y, V Molina, C Dorador, D Tapia. Virus en Salar de Huasco y su rol potencial sobre la vida microbiana planctónica y de tapetes microbianos en cuencas evaporíticas altiplánicas. IX Congreso de la Sociedad Chilena de Limnología, Antofagasta, 23 – 26 de Octubre de 2012, pág. 41. Peña H, H Levipan, C Dorador, C Cubillos, C Fernández, Y Eissler, M Hengst, K Hernandez, L Olsen y V Molina. Nitrificación, un proceso dinámico en el Salar de Huasco. IX Congreso de la Sociedad Chilena de Limnología, Antofagasta, 23 – 26 de Octubre de 2012, pág. 50. Kuznar J, Y Eissler, P Conejeros, MS Pavlov y JC Espinoza (2011). Virus IPN: Algunas inconsistencias en los resultados en el diagnóstico del virus mediante qRT-PCR con química Taqman. XXXIII Congreso Sociedad de Microbiología de Chile. Olmué, 29 de Noviembre - 2 de Diciembre de 2011. Eissler Y, MS Pavlov, P Conejeros, JC Espinoza & J Kuznar (2011). Caracterización Genética del Virus de la Necrosis Pancreática Infecciosa en Salmónidos del Sur de Chile. Resúmenes XXXI Congreso de Ciencias del Mar. Viña del Mar, Agosto 2011, pág. 95. Eissler Y, MS Pavlov, P Conejeros, JC Espinoza & J Kuznar (2011). Detección y Cuantificación del IPNV con un Ensayo Taqman en PCR Tiempo Real para la Región Codificadora de VP1. Resúmenes XXXI Congreso de Ciencias del Mar. Viña del Mar, Agosto 2011, pág. 94. Eissler Y, MS Pavlov, P Conejeros, JC Espinoza & J Kuznar (2010). Development of SYBR green I onestep real-time RT-PCR assay for detection and quantification of Infectious pancreatic necrosis virus and characterization of virulent Chilean IPNV strains. 22-26 November 2010 International conference: Environment and resources of the South Pacific, III International Aquaculture Congress, page 79. Eissler Y (2010). Marine viruses and their control on phytoplankton populations. Mini-Symposium of ECODIM VI. Departamento de Oceanografía, Universidad de Concepción, 15 de Enero de 2010. 7- PARTICIPATION INTERNATIONAL RESEARCH CRUISES 2004-2007, Eight Oceanographic Cruises Chesapeake Bay, Delaware Bay, USA, MOVE NSF Project. 2000, Two Oceanographic Cruises Skagerrak-Baltic Sea, Sweden. Wallenberg Foundation Project. 1995, March 25-April 3 Oceanographic Cruise Magellan Strait, Chile. Magellan Strait Joint Expedition Italian-Chilean. 1994, October 20-November 7 Oceanographic Cruise Magellan Strait and Beagle Channel, Chile. JOINT CHILEAN-GERMAN MAGELLAN EXPEDITION. 8- PARTICIPATION NATIONAL RESEARCH CRUISES 2002-2004, Participation in more than 15 cruises carried out in the northern (Iquique, Antofagasta) and south (Concepción) area of Chile, COPAS-FONDAP Center. 1992-1998, Eight Oceanographic Cruises, Coquimbo, Chile. Project JGOFS/SAREC. 1997-1998, Three Oceanographic Cruises, Antofagasta, Chile. Project Sectorial/Fondecyt 5960002-96. 1995-1996, Two Oceanographic Cruises, Arauco Golf, Concepción, Chile. Project FIP 95-11. 1991, Two Oceanographic Cruises: Fjords of Southern Chile. Project "Plankton Ecology". IRDC.