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ELECTRONIC SUPPLEMENTARY MATERIAL
PERforming a hepatic timing signal: glucocorticoids induce gper1a and gper1b
expression and repress gclock1a and gbmal1a in the liver of goldfish
Journal of Comparative Physiology. Part B.
Aída Sánchez-Bretaño, María Callejo, Marta Montero, Ángel L. Alonso-Gómez, María
J. Delgado, Esther Isorna*.
Departamento de Fisiología (Fisiología Animal II). Facultad de Biología. Universidad
Complutense de Madrid. 28040 Madrid, Spain.
* Corresponding author’s e-mail: eisornaa@bio.ucm.es
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APPENDIX 1
Relative expression (fold change)
2,5
2,5
gPer2a
2,5
gPer3
2,0
2,0
2,0
1,5
1,5
1,5
1,0
1,0
1,0
0,5
0,5
0,5
0,0
2,5
0,0
0,0
0
0.1
1
10 100
gCry1a
2,5
0
0.1
1
10 100
gCry2a
0
2,5
2,0
2,0
2,0
1,5
1,5
1,5
1,0
1,0
1,0
0,5
0,5
0,5
0,0
0,0
0 0.1 1 10 100
Dexamethasone (nM)
gClock2
0.1
1
10 100
gCry3
0,0
0 0.1 1 10 100
Dexamethasone (nM)
0 0.1 1 10 100
Dexamethasone (nM)
Fig. A1 Relative expression of gper2a, gper3, gcry1a, gcry2a, gcry3 and gclock2 in
goldfish cultured liver treated during 5 h with dexamethasone. Data obtained by
qRT-PCR are shown as the mean ± s.e.m. (n=6) in relative units (ΔΔCt method). No
statistically significant differences were found by one-way ANOVA for any of these
genes.
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